ABSTRACT
The effect of irrigation by sugar factory effluent (spentwash) and the well water from adjoining area has been studied in Wardha district, Maharashtra. The effluent had high TDS (422-608 mgL(-1)), COD (1152-17680 mgL(-1)) and BOD(380-650 mgL(-1)) than well water (TDS 240 mgL(-1), COD 3.8 mgL(-1) and BOD 1.2 mgL(-1)). There found some nutrients, viz. N, P, K, Zn, Cu, Fe, Mn in surface layer of soil in different seasons. Heavy metals (Cd, Co, Cr, Ni, Pb) were found to be within the permissible limits.
Subject(s)
Carbohydrates/chemistry , Conservation of Natural Resources , Environmental Monitoring/methods , Hazardous Waste , India , Industrial Waste , Industry , Metals, Heavy/analysis , Refuse Disposal/methods , Soil , Soil Pollutants/analysis , Trace Elements/analysis , Water/analysis , Water Pollutants, Chemical , Water SupplyABSTRACT
Allergic bronchopulmonary aspergillosis patients (ten in the age group 5-13) were studied with respect to clinical and immunodiagnostic tests. All the patients showed wheal and flare reaction with A. fumigatus antigens. Clinical features included bilateral infiltration and hilar lymphadenopathy. Eosinophilic count in these patients was observed to be in the range of 300-2500/mm3. Sera of six patients indicated precipitin reaction with A. fumigatus antigens. An elevated total serum IgE was noticed in all the patients. A. fumigatus specific IgG and IgE antibodies were increased in these patients. Optical density of the sera of all the patients against controls were observed to be in the range of (0.426 to 1.8 for IgG and 0.147 to 0.562 for IgE) by ELISA. Western blot analysis indicated that there may be correlation between the clinical stages of the disease and immunological reactivity of the sera with various antigenic components.
Subject(s)
Adolescent , Antibodies, Fungal/blood , Aspergillosis, Allergic Bronchopulmonary/blood , Aspergillus fumigatus/immunology , Child , Enzyme-Linked Immunosorbent Assay , Female , Humans , Immunoglobulin E/blood , Immunoglobulin G/blood , MaleABSTRACT
Metabolic antigens of Aspergillus fumigatus, soil strain 2605 and sputum isolate, were evaluated for their diagnostic applicability using hyperimmune sera and sera of adults and pediatric patients of allergic bronchopulmonary aspergillosis. An indirect ELISA was standardised by using 2-10 micrograms/ml of coating antigen for detection of specific IgG and IgE antibodies in the sera of patients. The ratios of absorbance for specific IgE and IgG antibodies by ELISA (normal to patients) were observed to be in the range of 1:2 to 1:3 to 1:8 respectively. These antigenic preparations were further analyzed to identify and characterize the individual components by immunoblotting. This analysis indicated the presence of allergenic and antigenic determinants in the antigens of molecular weights 70, 34, and 28 Kd. The utility of the antigens of soil strain for diagnostic purpose is suggested.